国产免费观看久久黄av片,亚洲人成网站观看在线播放 ,精品一区二区三区四区五区六区,国产农村妇女毛片精品久久

當(dāng)前位置:首頁  >  技術(shù)文章  >  TROAP在STAT3的幫助下促進腎透明細胞癌的增殖、遷移和轉(zhuǎn)移

TROAP在STAT3的幫助下促進腎透明細胞癌的增殖、遷移和轉(zhuǎn)移

更新時間:2024-12-29  |  點擊率:122

20236月,江南大學(xué)附屬醫(yī)院泌尿外科;南京醫(yī)科大學(xué)第一附屬醫(yī)院泌尿外科;江南大學(xué)無錫醫(yī)學(xué)院 (Department of Urology, Affiliated Hospital of Jiangnan University, Wuxi 214122, China;Department of Urology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210008, China;Wuxi Medical College, Jiangnan University, Wuxi 214122, China) Jun Wang老師研究團隊在《International Journal of Molecular Sciences》上發(fā)表論文:

 TROAP Promotes the Proliferation, Migration, and Metastasis of Kidney Renal Clear Cell Carcinoma with the Help of STAT3"

 

TROAPSTAT3的幫助下促進腎透明細胞癌的增殖、遷移和轉(zhuǎn)移"

 

Abstract

Kidney renal clear cell carcinoma (KIRC) is a subtype of renal cell carcinoma that threatens human health. The mechanism by which the trophinin-associated protein (TROAP)-an important oncogenic factor-functions in KIRC has not been studied. This study investigated the specific mechanism by which TROAP functions in KIRC. TROAP expression in KIRC was analyzed using the RNAseq dataset from the Cancer Genome Atlas (TCGA) online database. The Mann-Whitney U test was used to analyze the expression of this gene from clinical data. The Kaplan-Meier method was used for the survival analysis of KIRC. The expression level of TROAP mRNA in the cells was detected using qRT-PCR. The proliferation, migration, apoptosis, and cell cycle of KIRC were detected using Celigo, MTT, wound healing, cell invasion assay, and flow cytometry. A mouse subcutaneous xenograft experiment was designed to demonstrate the effect of TROAP expression on KIRC growth in vivo. To further investigate the regulatory mechanism of TROAP, we performed co-immunoprecipitation (CO-IP) and shotgun liquid chromatography-tandem mass spectrometry (LC-MS). TCGA-related bioinformatics analysis showed that TROAP was significantly overexpressed in KIRC tissues and was related to higher T and pathological stages, and a poor prognosis. The inhibition of TROAP expression significantly reduced the proliferation of KIRC, affected the cell cycle, promoted cell apoptosis, and reduced cell migration and invasion. The subcutaneous xenograft experiments showed that the size and weight of the tumors in mice were significantly reduced after TROAP-knockdown. CO-IP and post-mass spectrometry bioinformatics analyses revealed that TROAP may combine with signal transducer and activator of transcription 3 (STAT3) to achieve tumor progression in KIRC; this was verified by functional recovery experiments. TROAP may regulate KIRC proliferation, migration, and metastasis by binding to STAT3.


摘要:

腎透明細胞癌(KIRC)是一種嚴重威脅人類健康的腎細胞癌亞型。TROAP是一種重要的致癌因子,其在KIRC中的作用機制尚未得到研究。本研究探討了TROAPKIRC中的具體作用機制。使用來自癌癥基因組圖譜(TCGA)在線數(shù)據(jù)庫的RNAseq數(shù)據(jù)集分析TROAPKIRC中的表達。采用Mann-Whitney U檢驗從臨床資料中分析該基因的表達。采用Kaplan-Meier法進行KIRC的生存分析。采用qRT-PCR檢測細胞中TROAP mRNA的表達水平。采用Celigo、MTT、創(chuàng)面愈合、細胞侵襲試驗和流式細胞術(shù)檢測KIRC的增殖、遷移、凋亡和細胞周期。研究人員設(shè)計了小鼠皮下異種移植實驗來證明TROAP表達對KIRC體內(nèi)生長的影響。為了進一步研究TROAP的調(diào)控機制,研究人員采用了共免疫沉淀(CO-IP)和霰彈槍液相色譜-串聯(lián)質(zhì)譜(LC-MS)。tcga相關(guān)生物信息學(xué)分析顯示,TROAPKIRC組織中顯著過表達,與高T及病理分期、預(yù)后差有關(guān)。抑制TROAP表達可顯著降低KIRC的增殖,影響細胞周期,促進細胞凋亡,減少細胞遷移和侵襲。皮下異種移植實驗表明,敲除troap后,小鼠腫瘤的大小和重量明顯減小。CO-IP和質(zhì)譜后生物信息學(xué)分析顯示,TROAP可能與信號換能器和轉(zhuǎn)錄激活因子3 (STAT3)結(jié)合,在KIRC中實現(xiàn)腫瘤進展;功能恢復(fù)實驗證實了這一點。TROAP可能通過結(jié)合STAT3調(diào)控KIRC的增殖、遷移和轉(zhuǎn)移。

 

該論文中,對人KIRC細胞系786-O、ACHNCaki-1的體外培養(yǎng)是使用Ausbian特級胎牛血清完成的。


亚洲色欲色欲大片www无码| 日本最新免费二区三区| 日日碰狠狠躁久久躁综合网| 精品无人区乱码1区2区3区在线 | 欧美性xxxxx大屁股| 丰满少妇69激情啪啪无| 亚洲精品成人a在线观看| a级毛片无码久久精品免费| 国产精品亚洲一区二区无码| 精品久久久久久成人av| 在线观看大片免费播放器| 人妻放荡乱绿帽h文| 亚洲国产激情一区二区三区| 体验区试看120秒啪啪免费| 国产尤物AV尤物在线观看| 最近最新手机中文大全4| 肉大捧一进一出免费视频| 99久久人妻精品免费二区| 欧美性性性性性色大片免费的| 宅宅少妇无码| 精品国产精品久久一区免费式| 国产精品国产三级在线专区| 看av免费毛片手机播放| 亚洲人成精品久久久久| 国内精品久久久久精免费| 久久麻传媒亚洲av国产| 亲嘴伸进内衣揉胸口激烈视频| 亚洲国产精品久久久久秋霞影院| 欧美精品18VIDEOS性欧美| 国产成人无码免费视频在线| 国产AV人人夜夜澡人人爽麻豆 | 久久久久久九九99精品| 国产又爽又黄又爽又刺激| 亚洲精品无码久久久久sm| 亚洲xxxx做受欧美| 大肉大捧一进一出的视频| 亚洲av无码乱码国产精品fc2| 无码国产69精品久久久久孕妇| 亚洲熟女www一区二区三区| 精品无码国产一区二区三区AV| 久久久久久久精品国产亚洲|